Biochemical and Bioprocess Engineering Quiz Answers

Industrial Biotechnology

University of Manchester

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Biochemical and Bioprocess Engineering Quiz Answers

Question 1)

What is a suitable definition of a conserved quantity? 

• A quantity which is neither created nor destroyed “X”
• Something which accumulates over time
• A chemical species that is not involved in a particular chemical reaction

Question 2)

What is meant when a process is described as being “steady state”?

• All parameters are constant with respect to time “X” 
• Chemical equilibrium has been reached
• All variables are changing at a constant rate
• All molar flow rates are constant with respect to time

Question 3)

In microbial fermentation, which mode of operation corresponds to a steady state process?

• Fed-batch operation
• Batch operation
• Continuous operation “X”

Question 4)

In which phase of a cell culture would we expect to see the maximum growth rate? 

• Stationary
• Log “X”
• Acceleration
• Lag

Question 5)

Why would we need to include a homogeniser in a recovery process?

• Because we want to separate out contaminants on the basis of size
• Because the product is extracellular
• Because we want to separate out contaminants on the basis of density
• Because the product is intracellular “X”

Question 6)

Which of the following is not a type of chromatography used in bioprocessing?

• Thin layer “X”
• Hydrophobic interaction
• Ion exchange
• Size exclusion

Question 7)

Centrifugation separates particles on the basis of which property?

• Density “X”
• Hydrophobicity
• Size
• Charge

Question 8)

If a chromatography unit is used to capture a desired product whilst letting contaminants flow through, which mode is it operating in?

• Bind and elute “X”
• Size exclusion
• Flow through

Question 9)

What is the main idea and driver behind the use of biocatalysis?

• Availability of different modes of operation
• Cell growth and enzyme production are de-coupled from conversion
• Ease of integration with biorefineries

Question 10)

How many industrial biocatalytic processes are currently in operation?

• None
• Less than 10
• 50
• More than 150 “X”

Question 11)

What is the significance of yield as a fermentation metric in the context of the competitiveness of a process?

• Yield determines the maximum profit to be made
• Yield determines the capital cost of process equipment
• The process yield should be the same as the maximum theoretical yield

Question 12)

In a biocatalytic process how is yield defined?

• g product/L reactor volume/time
• g product/g biocatalyst
• g product /L reactor volume